Generator

Part:BBa_K1216004:Design

Designed by: Parvathi Chandran   Group: iGEM13_ETH_Zurich   (2013-08-29)


β-Glucuronidase (gusA) from Bacillis Subtilis with TEV and poly-HIS tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 536
    Illegal XhoI site found at 1861
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

add a his tag to enzyme


Source

E.coli

References